Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Zmiz1

Cell type

Cell type Class
Blood
Cell type
GM18946
Primary Tissue
Blood
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
8946: Flag-Zmiz1 Chromatin IP
cell line
8946 cells - Flag-Zmiz1/ L1601PDP
disease
T lymphoblastic leukemia/lymphoma
cell type
T lymphocyte
antibody
Flag (F1804, Sigma)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinking was performed with either 1% formaldehyde alone (histone mark samples) or 2 mM DSG and 1% formaldehyde ( Zmiz1/ICN1/Rbpj samples). Nuclear were sonicated and protein-DNA complexes were isolated with antibody. The sequencing library was prepared using the Illumina Hi-Seq library preparation kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
36147936
Reads aligned (%)
61.9
Duplicates removed (%)
12.8
Number of peaks
683 (qval < 1E-05)

mm9

Number of total reads
36147936
Reads aligned (%)
61.8
Duplicates removed (%)
12.9
Number of peaks
696 (qval < 1E-05)

Base call quality data from DBCLS SRA