Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Blood
Cell type
Lymphoblasts
NA
NA

Attributes by original data submitter

Sample

source_name
RAD21-ChIP-seq-WT
condition
Normal
cell type
Lymphoblast cells
antibody
RAD-21 (Abcam, catalog no. ab992)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Lymphoblastoid cells (1x10^7 cells) were fixed in phosphate-buffered saline (PBS) with 0.96% formaldehyde for 8 minutes at room temperature. Crosslinking was quenched with 125 mM Glycine. The cells were washed with PBS and precipitated via centrifugation. The cell pellet was stored in -80°C until use. ChIP-seq: Libraries were prepared using Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences, catalog no. 21024) according to manufacturer's protocol. 4C-seq: 4C-seq was performed and ibraries were prepared according to standard procedures (Krijger et al. Methods 170, 17–32 (2020))

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
86316208
Reads aligned (%)
69.4
Duplicates removed (%)
26.6
Number of peaks
24253 (qval < 1E-05)

hg38

Number of total reads
86316208
Reads aligned (%)
72.1
Duplicates removed (%)
25.7
Number of peaks
23550 (qval < 1E-05)

Base call quality data from DBCLS SRA