Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
JUN

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-231 breast cancer cells
cell line
MDA-MB-231
chip antibody
JUN (BD Bioscience, 610326, lot 3263734)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sheared by sonication and immunoprecipitated with YAP, TAZ, TEAD4 and JUN antibodies. DNA was purified by phenol/chloroform extraction and ethanol precipitation. NuGEN Ovation Ultralow Library Prep System kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
85747303
Reads aligned (%)
98.1
Duplicates removed (%)
10.2
Number of peaks
37569 (qval < 1E-05)

hg19

Number of total reads
85747303
Reads aligned (%)
97.4
Duplicates removed (%)
11.5
Number of peaks
37275 (qval < 1E-05)

Base call quality data from DBCLS SRA