Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TEAD4

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-231 breast cancer cells
cell line
MDA-MB-231
chip antibody
TEF-3 (N-G2) (SCBT, sc-101184, lot C2812)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sheared by sonication and immunoprecipitated with YAP, TAZ, TEAD4 and JUN antibodies. DNA was purified by phenol/chloroform extraction and ethanol precipitation. NuGEN Ovation Ultralow Library Prep System kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
114856794
Reads aligned (%)
97.4
Duplicates removed (%)
16.6
Number of peaks
7626 (qval < 1E-05)

hg19

Number of total reads
114856794
Reads aligned (%)
96.6
Duplicates removed (%)
18.2
Number of peaks
6979 (qval < 1E-05)

Base call quality data from DBCLS SRA