Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
WWTR1

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-231 breast cancer cells
cell line
MDA-MB-231
chip antibody
WWTR1 (Sigma, HPA007415,lot C78623)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sheared by sonication and immunoprecipitated with YAP, TAZ, TEAD4 and JUN antibodies. DNA was purified by phenol/chloroform extraction and ethanol precipitation. NuGEN Ovation Ultralow Library Prep System kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
90359824
Reads aligned (%)
97.4
Duplicates removed (%)
26.4
Number of peaks
9239 (qval < 1E-05)

hg19

Number of total reads
90359824
Reads aligned (%)
96.6
Duplicates removed (%)
28.1
Number of peaks
8923 (qval < 1E-05)

Base call quality data from DBCLS SRA