Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Blood
Cell type
Mono-Mac-6
NA
NA

Attributes by original data submitter

Sample

source_name
Monomac6 cell line
cell line
Monomac6
cell type
AML
genome variation
CTRL
chip antibody
H3K4me1 (abcam ab8895)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Briefly, samples were crosslinked, lysed, sonicated and immunoprecipated with the indicated antibody overnight. After a series of washes they were reverse crosslinked overnight and DNA extracted. Library prep was performed at Genewiz, Illumina HiSeq, 2x150bp paired-end reads, multiplexed

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
35098603
Reads aligned (%)
90.2
Duplicates removed (%)
8.5
Number of peaks
2424 (qval < 1E-05)

hg19

Number of total reads
35098603
Reads aligned (%)
89.6
Duplicates removed (%)
8.7
Number of peaks
1862 (qval < 1E-05)

Base call quality data from DBCLS SRA