Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

MOLM-13

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Molm13 cell line

cell line

Molm13

cell type

AML

genome variation

CTRL

chip antibody

H3K27ac (abcam ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Briefly, samples were crosslinked, lysed, sonicated and immunoprecipated with the indicated antibody overnight. After a series of washes they were reverse crosslinked overnight and DNA extracted. Library prep was performed at Genewiz, Illumina HiSeq, 2x150bp paired-end reads, multiplexed

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

33800893

Reads aligned (%)

88.3

Duplicates removed (%)

10.3

Number of peaks

13945 (qval < 1E-05)

hg19

Number of total reads

33800893

Reads aligned (%)

87.8

Duplicates removed (%)

10.4

Number of peaks

13553 (qval < 1E-05)