Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MED1

Cell type

Cell type Class
Blood
Cell type
AMU-AML1
NA
NA

Attributes by original data submitter

Sample

source_name
AMU-AML1 cell line
cell line
AMU-AML1
cell type
AML
genome variation
MN1-ETV6
chip antibody
Mediator1 (Bethyl A300-793A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Briefly, samples were crosslinked, lysed, sonicated and immunoprecipated with the indicated antibody overnight. After a series of washes they were reverse crosslinked overnight and DNA extracted. Library prep was performed at Genewiz, Illumina HiSeq, 2x150bp paired-end reads, multiplexed

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
33855275
Reads aligned (%)
80.6
Duplicates removed (%)
30.6
Number of peaks
330 (qval < 1E-05)

hg38

Number of total reads
33855275
Reads aligned (%)
82.1
Duplicates removed (%)
30.2
Number of peaks
730 (qval < 1E-05)

Base call quality data from DBCLS SRA