Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
Peripheral blood stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
mobPBSC
cell type
PBSC
antibody target
H3K4me3
company
Abcam
catalogue number
ab8580
reference genome
HG19

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sonicated and used for ChIP seq using specific antibodies (avialable on suplementry table). ChIP seq libraries were preapared according to Illumina's standard protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
15420100
Reads aligned (%)
96.2
Duplicates removed (%)
63.5
Number of peaks
24010 (qval < 1E-05)

hg19

Number of total reads
15420100
Reads aligned (%)
95.5
Duplicates removed (%)
64.5
Number of peaks
23790 (qval < 1E-05)

Base call quality data from DBCLS SRA