Antigen

Antigen Class

TFs and others

Antigen

ZNF165

Cell type

Cell type Class

Breast

Cell type

WHIM12

NA

NA

Sample

source_name

breast cancer tumor derived cell line stably expressing ZNF165-V5

tumor type

TNBC

cell line

WHIM12 stably expressing ZNF165-V5

chip antibody

Polyclonal V5 Antibody (Abcam, catalog# 9116)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei from WHIM12 cells stably expressing control or ZNF165-V5 were isolated, sonicated and immunopreciptated with V5 antibody. 5 ng of ChIP-ed DNA underwent library preparation using the KAPA HTP Library Preparation Kit. ChIP-ed DNA was end repaired, 3’ end adenylated and barcoded with multiplex adaptors. Ampure XP beads were used to purify DNA. Library size was assessed using the Agilent 2100 Bioanalyzer. The Invitrogen Qubit® Fluorometer was then used to quantify samples prior to normalizing and pooling.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

34872750

Reads aligned (%)

96.9

Duplicates removed (%)

10.6

Number of peaks

1098 (qval < 1E-05)

hg19

Number of total reads

34872750

Reads aligned (%)

96.2

Duplicates removed (%)

11.9

Number of peaks

974 (qval < 1E-05)