GSM1611458: WHIM12 ZNF165 Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
WHIM12
NA
NA
Attributes by original data submitter
Sample
source_name
breast cancer tumor derived cell line stably expressing ZNF165-V5
tumor type
TNBC
cell line
WHIM12 stably expressing ZNF165-V5
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei from WHIM12 cells stably expressing control or ZNF165-V5 were isolated, sonicated and immunopreciptated with V5 antibody. 5 ng of ChIP-ed DNA underwent library preparation using the KAPA HTP Library Preparation Kit. ChIP-ed DNA was end repaired, 3’ end adenylated and barcoded with multiplex adaptors. Ampure XP beads were used to purify DNA. Library size was assessed using the Agilent 2100 Bioanalyzer. The Invitrogen Qubit® Fluorometer was then used to quantify samples prior to normalizing and pooling.