Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Pou2f1

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD4 T cells
strain
C57BL/6
tissue
Spleen
age
postnatal 8 weeks
treatment
restimulated
antibody
Oct1
antibody vendor/catalog
SantaCruz, catalog# sc-232X

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and trancription factor-DNA complexes were isolated with antibody. ChIP DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
58936118
Reads aligned (%)
69.9
Duplicates removed (%)
13.5
Number of peaks
19187 (qval < 1E-05)

mm9

Number of total reads
58936118
Reads aligned (%)
69.7
Duplicates removed (%)
13.5
Number of peaks
19199 (qval < 1E-05)

Base call quality data from DBCLS SRA