GSM4672361: ChIP-seq H3K4me1 in human aortic valve interstitial cells (input); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Cardiovascular
Cell type
Aortic valve interstitial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Human aortic valve interstitial cells from non-mineralized aortic valves
cell type
aortic valve interstitial cells
antibody
input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Human aortic valve interstitial cells were isolated from control non-mineralized aortic valves obtained from patients undergoing heart transplantation. The protocol was approved by the local ethical committee and informed consent was obtained from the subjects. Aortic leaflets were cut into pieces and incubated in 0.3% type I collagenase (Invitrogen, Thermo Fisher Scientific, ON, Canada) at 37°C for 45 minutes, then filtered through a 70 µm mesh, centrifuged for 5 minutes at 1,500 rpm and resuspended in complete media (DMEM, 10% FBS with L-glutamine and sodium pyruvate). Cells were used between passages 3 to 7. Nuclear extracts from VICs were immunoprecipitated with antibodies against H3K4me3, H3K4me1 (Cell Signaling Technology, New England Biolabs, ON, Canada) or H3K27Ac (Abcam, ON, Canada) pre-bound to protein G Dynabeads (Life Technologies, Thermo Fisher Scientific, ON, Canada). DNA was purified using DNA clean up & concentrator kit (Zymo Research, Cedarlane, Canada). Libraries were constructed using the QIAseq Ultralow Input Library Kit (Qiagen, ON, Canada) according to manufacturer's instructions. Sequencing was performed on an Illumina HiSeq4000 (UCSD IGM Genomics Facility, CA, USA).