GSM4668329: input MCF7 estradiol Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
input MCF7 estradiol
cell line
MCF7
source tissue
mammary gland, breast; derived from metastatic site: pleural effusion
treatment
Estradiol 10 nM 1h
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MCF7 cells were crosslinked, chromatin from nuclear lisates was sonicated and protein-DNA complexes were isolated with antibody. Input samples correspond to 10% of the material before antibody isolation. Libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina according to the manufacturer's instructions. NEBNext Multiplex Oligos for Illumina were used during library amplification