Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
E(z)

Cell type

Cell type Class
Cell line
Cell type
Cultured cell lines
NA
NA

Attributes by original data submitter

Sample

source_name
Cultured cells
cell line
Psc4-1
mutation
Su(z)2-1.b8
antibody
E(Z) from Kahn et al., 2006

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Live cells were crosslinked with 1.8% formaldehyde for 10 min at 25C and soluble chromatin prepared as described in Schwartz et al., 2006. ChIP was done as described in Schwartz et al., 2006. Libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set and Multiplex Oligos for Illumina (New England BioLabs) following the manufacturer instructions.

Sequencing Platform

instrument_model
Illumina MiSeq

dm3

Number of total reads
7594510
Reads aligned (%)
95.0
Duplicates removed (%)
5.6
Number of peaks
7319 (qval < 1E-05)

dm6

Number of total reads
7594510
Reads aligned (%)
94.9
Duplicates removed (%)
6.2
Number of peaks
7643 (qval < 1E-05)

Base call quality data from DBCLS SRA