Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Liver
Cell type
Fetal liver
NA
NA

Attributes by original data submitter

Sample

source_name
Fetal_liver_H3K27me3_H3K4me3_Sequential
developmental stage
12 weeks after gestation
tissue
fetal liver
chip antibody
H3K27me3 (CST, #9733)
chip antibody
H3K4me3 (Abcam, ab8580)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPed DNA was purified by phenol chloroform, ethanol-precipitated and was further resuspended in 10 mM Tris-HCl pH 8.0. ChIP sequencing libraries were constructed under the standard protocol of the NEBNext® Ultra™ DNA Library Prep Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
12167577
Reads aligned (%)
92.2
Duplicates removed (%)
4.7
Number of peaks
382 (qval < 1E-05)

hg19

Number of total reads
12167577
Reads aligned (%)
91.7
Duplicates removed (%)
4.7
Number of peaks
377 (qval < 1E-05)

Base call quality data from DBCLS SRA