Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Neural
Cell type
Fetal brain
NA
NA

Attributes by original data submitter

Sample

source_name
Fetal_brain_H3K4me3
developmental stage
12 weeks after gestation
tissue
fetal brain
chip antibody
H3K4me3
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPed DNA was purified by phenol chloroform, ethanol-precipitated and was further resuspended in 10 mM Tris-HCl pH 8.0. ChIP sequencing libraries were constructed under the standard protocol of the NEBNext® Ultra™ DNA Library Prep Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
15083468
Reads aligned (%)
91.0
Duplicates removed (%)
2.0
Number of peaks
21540 (qval < 1E-05)

hg19

Number of total reads
15083468
Reads aligned (%)
90.5
Duplicates removed (%)
2.0
Number of peaks
21348 (qval < 1E-05)

Base call quality data from DBCLS SRA