Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Neural
Cell type
Fetal brain
NA
NA

Attributes by original data submitter

Sample

source_name
Fetal_brain_H3K27me3_H3K4me3_Sequential
developmental stage
12 weeks after gestation
tissue
fetal brain
chip antibody
H3K27me3 (CST, #9733)
chip antibody
H3K4me3 (Abcam, ab8580)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPed DNA was purified by phenol chloroform, ethanol-precipitated and was further resuspended in 10 mM Tris-HCl pH 8.0. ChIP sequencing libraries were constructed under the standard protocol of the NEBNext® Ultra™ DNA Library Prep Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
3995701
Reads aligned (%)
78.6
Duplicates removed (%)
5.5
Number of peaks
80 (qval < 1E-05)

hg19

Number of total reads
3995701
Reads aligned (%)
77.9
Duplicates removed (%)
5.6
Number of peaks
83 (qval < 1E-05)

Base call quality data from DBCLS SRA