Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Kasumi-1 Cells
chip antibody
anti-AML1 (N20) (sc-8563 X; Santa Cruz Biotechnology)
cell line
AML1-ETO bearing AML patient cell lines Kasumi-1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP and amplification were performed according to the Affymetrix protocol as described (Carroll et al., 2005).Total input DNA was extracted from sonicated nuclei. Protein-DNA complexes were isolated with specific antibodies and protein A/G beads. The following antibodies were used for protein-DNA complexes isolation: anti-ETO antibody (sc9737 X) that recognizes AML1/ETO, anti-AML1 (C19) antibody (ab23980) that recognizes AML1, anti-AML1 (N20) antibody (sc8563 X) that recognizes both AML1/ETO and AML1. Libraries were prepared according to the Illumina genomic prep kit (Illumina, San Diego, CA, USA)

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
18368604
Reads aligned (%)
85.9
Duplicates removed (%)
2.8
Number of peaks
14087 (qval < 1E-05)

hg38

Number of total reads
18368604
Reads aligned (%)
87.5
Duplicates removed (%)
1.9
Number of peaks
14013 (qval < 1E-05)

Base call quality data from DBCLS SRA