Antigen

Antigen Class

TFs and others

Antigen

RUNX1

Cell type

Cell type Class

Blood

Cell type

Kasumi-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Kasumi-1 Cells

chip antibody

anti-AML1 (C19) (ab23980; Abcam)

cell line

AML1-ETO bearing AML patient cell lines Kasumi-1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP and amplification were performed according to the Affymetrix protocol as described (Carroll et al., 2005).Total input DNA was extracted from sonicated nuclei. Protein-DNA complexes were isolated with specific antibodies and protein A/G beads. The following antibodies were used for protein-DNA complexes isolation: anti-ETO antibody (sc9737 X) that recognizes AML1/ETO, anti-AML1 (C19) antibody (ab23980) that recognizes AML1, anti-AML1 (N20) antibody (sc8563 X) that recognizes both AML1/ETO and AML1. Libraries were prepared according to the Illumina genomic prep kit (Illumina, San Diego, CA, USA)

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

18055494

Reads aligned (%)

89.5

Duplicates removed (%)

1.3

Number of peaks

14299 (qval < 1E-05)

hg19

Number of total reads

18055494

Reads aligned (%)

88.8

Duplicates removed (%)

2.1

Number of peaks

14390 (qval < 1E-05)