Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9ac

Cell type

Cell type Class
Blood
Cell type
Bone marrow mononuclear cells
NA
NA

Attributes by original data submitter

Sample

source_name
Bone marrow mononuclear cells
diagnosis
Acute myeloid leukemia (AML)
patient
REB20
cell type
Bone marrow mononuclear cells
treatment
DMSO
chip antibody
H3K9ac (Active Motif, Carlsbad, CA)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq libraries were prepared using SMARTer ThruPLEX DNA-seq kit (Takara Bio USA, Mountain View, CA) according to the manufacturer's instructions. 1 to 2 ng fragmented double stranded DNAs were used to construct libraries with dual indices.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg38

Number of total reads
17343426
Reads aligned (%)
94.2
Duplicates removed (%)
5.1
Number of peaks
7173 (qval < 1E-05)

hg19

Number of total reads
17343426
Reads aligned (%)
92.8
Duplicates removed (%)
6.6
Number of peaks
8021 (qval < 1E-05)

Base call quality data from DBCLS SRA