Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Toledo

Primary Tissue

Blood

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Lymphoma B-cell

Sample

source_name

Toledo_H3K4me3_DMSO

cell line

Toledo lymphoma cell line

treatment

DMSO

chip antibody

H3K4me3 (abcam ab8580)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated chromatin was immunoprecipitated using the ChIP antibodies indicated Libraries were made using the NEBNext Ultra II DNA library prep kit

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

42602691

Reads aligned (%)

95.9

Duplicates removed (%)

18.3

Number of peaks

13452 (qval < 1E-05)

hg19

Number of total reads

42602691

Reads aligned (%)

95.0

Duplicates removed (%)

19.6

Number of peaks

13426 (qval < 1E-05)