Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Toledo
Primary Tissue
Blood
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Lymphoma B-cell

Attributes by original data submitter

Sample

source_name
Toledo_H3K27ac_DMSO
cell line
Toledo lymphoma cell line
treatment
DMSO
chip antibody
H3K27ac (abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated chromatin was immunoprecipitated using the ChIP antibodies indicated Libraries were made using the NEBNext Ultra II DNA library prep kit

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg38

Number of total reads
40798275
Reads aligned (%)
96.3
Duplicates removed (%)
16.7
Number of peaks
907 (qval < 1E-05)

hg19

Number of total reads
40798275
Reads aligned (%)
95.3
Duplicates removed (%)
17.9
Number of peaks
1047 (qval < 1E-05)

Base call quality data from DBCLS SRA