Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Breast

Cell type

BT-549

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

Breast Cancer

cell line

BT-549

cell line type

basal-like breast cancer

chip antibody

H3K27Ac

chip antibody vendor

Abcam

young_id

20140911_3051

tissue

Breast

cell number

50 million

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Whole cell extracts were sonicated to solubilize the chromatin.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

40080982

Reads aligned (%)

93.1

Duplicates removed (%)

11.4

Number of peaks

38914 (qval < 1E-05)

hg19

Number of total reads

40080982

Reads aligned (%)

92.8

Duplicates removed (%)

11.8

Number of peaks

38977 (qval < 1E-05)