Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
S2-DRSC

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
S2-DRSC cell line
strain
DRSC
cell line
S2
chip antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Su(H)-DNA complexes were isolated with antibody. Sequencing libraries were prepared with the NEB Next ChIP-Seq Library Prep Kit according to NEB’s instructions (New England Biolabs, Ipswich, MA, USA). After adapter ligation, library fragments of 250-350 bp were isolated and PCR amplified with 18 cycles. Library fragments were purified, loaded on an Illumina flow cell for cluster generation and sequenced on the HiSeq 2000 (TruSeq cBot-HS v3 and TruSeq SBS Kit v3) following the manufacturer’s protocols (Illumina, San Diego, CA, USA)

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
30505252
Reads aligned (%)
94.2
Duplicates removed (%)
16.6
Number of peaks
4193 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA