Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2-DRSC cell line
strain
DRSC
cell line
S2
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Su(H)-DNA complexes were isolated with antibody. Sequencing libraries were prepared with the NEB Next ChIP-Seq Library Prep Kit according to NEB’s instructions (New England Biolabs, Ipswich, MA, USA). After adapter ligation, library fragments of 250-350 bp were isolated and PCR amplified with 18 cycles. Library fragments were purified, loaded on an Illumina flow cell for cluster generation and sequenced on the HiSeq 2000 (TruSeq cBot-HS v3 and TruSeq SBS Kit v3) following the manufacturer’s protocols (Illumina, San Diego, CA, USA)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
30505252
Reads aligned (%)
94.0
Duplicates removed (%)
19.6
Number of peaks
3868 (qval < 1E-05)

dm3

Number of total reads
30505252
Reads aligned (%)
94.2
Duplicates removed (%)
16.6
Number of peaks
4193 (qval < 1E-05)

Base call quality data from DBCLS SRA