Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MIF

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa
treatment
50 µM MNNG for 15 min, returned to fresh media for 4h before harvest.
chip antibody
Goat polyclonal anti-MIF(Abcam ab38146)
cell line
HeLa

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and MIF-DNA complexes were isolated with antibody. Libraries were prepared with Illumina Truseq DNA LT Sample Prep Kit with 15 cycles of PCR amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
86884954
Reads aligned (%)
67.5
Duplicates removed (%)
26.9
Number of peaks
1435 (qval < 1E-05)

hg38

Number of total reads
86884954
Reads aligned (%)
68.3
Duplicates removed (%)
26.2
Number of peaks
2119 (qval < 1E-05)

Base call quality data from DBCLS SRA