GSM1587485: DMSO treated MIF IP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MIF
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
HeLa
treatment
DMSO(0.1%) for 15 min, returned to fresh media for 4h before harvest.
chip antibody
Goat polyclonal anti-MIF(Abcam ab38146)
cell line
HeLa
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and MIF-DNA complexes were isolated with antibody. Libraries were prepared with Illumina Truseq DNA LT Sample Prep Kit with 15 cycles of PCR amplification.