Curated Sample Data


Genome
hg19
Antigen Class
TFs and others
Antigen
EZH2
Cell type Class
Digestive tract
Cell type
Het-1A

Cell type information


Primary Tissue
Esophagus
Tissue Diagnosis
Normal

Attributes by Original Data Submitter


source_name
Het-1A
tissue
Immortalized normal esophageal epithelium
chip antibody
EZH2

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Platform Information


instrument_model
Illumina HiSeq 4000

External Database Query

Logs in read processing pipeline


Number of total reads
19624415
Reads aligned (%)
34.7
Duplicates removed (%)
3.9
Number of peaks
121 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA