Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
iPSC
clone
WT_B
differentiation
Undifferentiated
treatment
Triptolide
molecule subtype
DNA pulldown by EZH2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA or RNA samples in fRIP-seq were extracted with Trizol following manufactuer's protocol. DNA samples in ChIP-seq was extracted using phenol-chloroform. KAPA RNA Hyperprep kit with riboerase (RNA-seq and fRIP-seq), KAPA HyperPlus Kit (ChIP-seq)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
13644729
Reads aligned (%)
87.2
Duplicates removed (%)
7.6
Number of peaks
649 (qval < 1E-05)

hg38

Number of total reads
13644729
Reads aligned (%)
89.4
Duplicates removed (%)
6.1
Number of peaks
681 (qval < 1E-05)

Base call quality data from DBCLS SRA