Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD4+CXCR5+CD62Llo T cells
strain
C57BL/6
genotype/variation
Utx fl/fl Lck-Cre
Stage
Day 21 post infection
tissue
Spleen
cell type
CD4+CXCR5+CD62Llo T cells
chip antibody
Abcam ab6002 anti-H3K27me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed for Chromatin Imunoprecipitation. DNA was end repaired, A-tailed, then Illumina barcode adaptors were ligated and amplified.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
26296915
Reads aligned (%)
97.8
Duplicates removed (%)
88.9
Number of peaks
158 (qval < 1E-05)

mm9

Number of total reads
26296915
Reads aligned (%)
97.6
Duplicates removed (%)
89.0
Number of peaks
169 (qval < 1E-05)

Base call quality data from DBCLS SRA