Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Uterus
Cell type
Endometrial stromal cells
NA
NA

Attributes by original data submitter

Sample

source_name
Isolated stromal cells post-hysterectomy
cell type
Primary endometrial stromal cells
individual
Patient 1
antibody target
ESR1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
7.5e8 cells (patient number = 1) were used for ChIP-Seq using the SimpleChIP Kit (Cell Signaling Technology, #9005). ESR1 antibody was purchased from Santa Cruz (#sc-543). Next-generation sequencing libraries were prepared using the KAPA Hyper Prep Kit (KAPA Biosystems, KK8502) and KAPA Single-Indexed Adapter Kit (KAPA Biosystems, KK8710).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
24004585
Reads aligned (%)
90.9
Duplicates removed (%)
8.4
Number of peaks
446 (qval < 1E-05)

hg38

Number of total reads
24004585
Reads aligned (%)
92.9
Duplicates removed (%)
7.1
Number of peaks
319 (qval < 1E-05)

Base call quality data from DBCLS SRA