Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAZ

Cell type

Cell type Class
Lung
Cell type
NCI-H1299
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Non-Small Cell

Attributes by original data submitter

Sample

source_name
H1299 cells
cell type
non-small cell lung cancer
cell line
H1299
antibodies
Anti-TAZ (Proteintech Cat: 23306-1-AP, RRID:AB_2721185)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei. The chromatin solution was immunoprecipitated with either YAP antibody or TAZ antibody. The immunoprecipitations were performed using Pierce ChIP-grade Protein G magnetic beads (Thermo Fisher Scientific). Reverse cross-linking and DNA purification was performed by ChIP DNA Clean & Concentrator kit (Zymo Research, USA). Libraries were prepared as described in Blecher-Gonen et al., Nat Protoc, 2013. 8(3): p. 539-54.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
32636127
Reads aligned (%)
47.7
Duplicates removed (%)
48.0
Number of peaks
3262 (qval < 1E-05)

hg38

Number of total reads
32636127
Reads aligned (%)
52.1
Duplicates removed (%)
44.3
Number of peaks
3215 (qval < 1E-05)

Base call quality data from DBCLS SRA