Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

8w Mouse Testis

strain

C57BL/6, Mixed

developmental stage

8 weeks

tissue

Testes

antibody

none (input)

antibody

none (input)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Testis samples were fixed with formaldehyde. The ChIP libraries were made from the input and the material immuniprecipitated with the Dmrt1 antibody described in PMID 11040213. Chromatin fragments were blunted with Lucigen DNA terminator enyme mix (Lucigen# 40035). Blunted fragments were adenylated with Exo- Klenow (NEB #MO2125). Illumina adapters (Illumina #100521) were diluted 1:50 and ligated to adenylated fragments with T4 ligase (NEB #NEB M0202T). Products were amplified with 18 rounds of PCR using Phusion Polymerase (NEB M0530s) using barcoded oligonucleotides. DNA fragments between 150 and 250 were purified by agarose gel electrophoresis. ChIP-Exo libraries were prepared as described in PMID: 22153082.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

31189059

Reads aligned (%)

98.4

Duplicates removed (%)

11.7

Number of peaks

478 (qval < 1E-05)

mm9

Number of total reads

31189059

Reads aligned (%)

98.2

Duplicates removed (%)

11.7

Number of peaks

535 (qval < 1E-05)