GSM4566014: H3K27me3 - HPC donor CB2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Blood
Cell type
CD34+
NA
NA
Attributes by original data submitter
Sample
source_name
Human cord blood CD34+
tissue
cord blood CD34+
chip antibody
H3K27me3 (Diagenode, C15200152)
cell population
HPC
donor
CB D2
sort phenotype
CD34+ CD3/14/19/56-
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
50-100 000 sorted, fixed cells were lysed in lysis buffer complemented with 20mM NaBU HDAC inhibitor and sheared on the Bioruptor Pico (Diagenode). Sheared chromatin was magnetically immunoprecipitated and tagmentated using the Auto ChIPmentation Kit for Histones (Diagenode). Libraries were constructed with the Auto ChIPmentation Kit for histones (Diagenode). Libraries were sequenced with the Nextseq500 (SR75, High Output).