Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Brd2

Cell type

Cell type Class
Blood
Cell type
Erythroblasts
MeSH Description
Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.

Attributes by original data submitter

Sample

source_name
GATA1 null erythroblasts
cell type
GATA1 null erythroblasts
gata1 transgene
GATA1-ER
treatment
100nM estradiol for 24 hours
antibody
Bethyl BRD2 A302-583A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed as described (Kadauke et al. Cell 2012). Cells were fixed in 1% formaldehyde for 10 minutes with gentle shaking at room temperature. Sonication employed a Diagenode Bioruptor (High signal, 30 seconds on/30 seconds off, for 30 minutes). IPs were performed using protein A/G sepharose beads. Beads were washed in high salt buffer and eluted in buffer containing bicarbonate and SDS. Crosslinks were reversed by heating to 65 degrees overnight. DNA was purified on Qiagen miniprep columns. Libraries were prepared using the protocol as outlined for Illumina's TruSeq ChIP Sample Prep Kit ( IP-202-1012), except that the libraries were size selected using Agencourt SPRIselect beads for an average size of ~300-325bp prior to PCR amplification. Library quality was assessed using the Aglient Bioanalzyer 2100 and libraries were sequenced on the Illumina HiSeq2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
50228655
Reads aligned (%)
92.8
Duplicates removed (%)
28.7
Number of peaks
11332 (qval < 1E-05)

mm9

Number of total reads
50228655
Reads aligned (%)
92.5
Duplicates removed (%)
28.6
Number of peaks
11790 (qval < 1E-05)

Base call quality data from DBCLS SRA