Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Cardiovascular
Cell type
HCASMC
NA
NA

Attributes by original data submitter

Sample

source_name
HCASMC_Input ChIP-Seq (for AHR ChIP)
cell line
Human Coronary Artery Smooth Muscle Cells (HCASMC)
tissue
coronary artery
genotype/treatment
dioxin (TCDD) 10nM

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked DNA from HCASMC between passages 5-8 in the presence of 10nM of TCDD was sonicated and extracted with Zymo DNA extraction kit, and library was constructed using the KAPA Hyper Prep Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
17289333
Reads aligned (%)
81.4
Duplicates removed (%)
7.2
Number of peaks
1800 (qval < 1E-05)

hg19

Number of total reads
17289333
Reads aligned (%)
79.7
Duplicates removed (%)
8.0
Number of peaks
923 (qval < 1E-05)

Base call quality data from DBCLS SRA