Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CBP

Cell type

Cell type Class
Adult
Cell type
Mushroom Bodies
MeSH Description
Prominent lobed neuropils found in ANNELIDA and all ARTHROPODS except crustaceans. They are thought to be involved in olfactory learning and memory.

Attributes by original data submitter

Sample

source_name
mushroom body
line
CS10
tissue
mushroom body
treatment
5-min optogenetic activation
chip antibody
anti-CBP (lab made antibodies)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Heads were collected and homogenized in crosslinking buffer. The nuclei were dissolved in extraction buffer, sonicated to dissociate the individual nuclei, and precipitated with ANTI-FLAG M2 Affinity Gel. The precipitates were rinsed 4 times in extraction buffer, with 5 min nutation at 4℃ between washes, and subjected to ChIP analysis or RNA extraction for RNA-seq. The library for ChIP-seq was prepared by NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs inc. Ipswich, MA, USA), and that for nuclear RNA-seq was prepared by Oligo d(T)25 Magnetic Beads (New England Biolabs inc. Ipswich, MA, USA), and NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs inc. Ipswich, MA, USA), according to the manufacturer's instructions.

Sequencing Platform

instrument_model
HiSeq X Ten

dm3

Number of total reads
22217415
Reads aligned (%)
72.9
Duplicates removed (%)
51.2
Number of peaks
7070 (qval < 1E-05)

dm6

Number of total reads
22217415
Reads aligned (%)
70.8
Duplicates removed (%)
52.4
Number of peaks
5307 (qval < 1E-05)

Base call quality data from DBCLS SRA