Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
LEF1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1 human ESCs
cell type
embryonic stem cells
passages/media
40-50/mTeSR
treatment
untreated
antibody
LEF-1 (homemade)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIPseq, DNA was extracted Qiaquick PCR extraction kit (cat 28106, Qiagen). For GROseq, nascent RNA purification was done using Trizol LS (Invitrogene). For ChIPseq, libraries were done with Illumina reagents. For GROseq, libraries were prepared with NEBnext kit (E7300S)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
7538195
Reads aligned (%)
92.7
Duplicates removed (%)
2.3
Number of peaks
330 (qval < 1E-05)

hg38

Number of total reads
7538195
Reads aligned (%)
93.6
Duplicates removed (%)
1.9
Number of peaks
296 (qval < 1E-05)

Base call quality data from DBCLS SRA