GSM4547023: INPUT DNA COMBO; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
PC3 prostate cancer cells
sample type
cell line derived from prostate cancer metastasis
antibody
none
treatment
combined HDAC/EZH2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked for 12 min at room temperature by the addition of one-tenth of the volume of 11% formaldehyde solution to the growth media. DNA was prepared using Agilent Chip-on-chip protocol (G4481-90010). Each immunoprecipitation was performed by incubating 10ug of the corresponding antibody with sheared DNA overnight at 4 degrees C, followed by 2 hour incubation with protein A/G (50/50) dynabeads and magnetic isolation of beads. Cross-links were reversed, and purified DNA was sent to the Dana Farber Center for Computation Cancer Biology (CCCB) core for library construction and sequencing. Performed by Dana Farber CCCB using NEBNext ChIP Master Mix Kit for Illumina following manufacturers instructions.