Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT5B

Cell type

Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Pre-activated CD8+ T cells
tissue
Peripheral blood
cell type
Pre-activated CD8+ T cells
chip antibody
STAT5B (Invitrogen, 135300, LOT-1477602A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA-Seq, RNA from 5 donors was pooled, and 1 μg of the pooled RNA was used to synthesize cDNA 220-400 bp fragments were isolated using 2% E-Gel (Invitrogen), ends were repaired, adaptor was added using T4 DNA ligase (New England Biolabs), and amplified for 17 cycles using PE 1.0 and PE 2.0 primers (Illumina) and Phusion High Fidelity PCR Master Mix (New England Biolabs). For ChIP-Seq, Chromatin from 10-20 million cells was sonicated into 250-500 bp fragments, DNA ends were repaired using polynucleotide kinase and Klenow enzyme, and treated with Taq polymerase to generate a protruding 3' “A” nucleotide. DNA fragments of ~250-450 bp were purified from agarose gels and used for cluster generation and sequencing. Antibody against STAT5B was used for immunoprecipitation. PCR products were barcoded (indexed) and sequenced on Illumina HiSeq 2000 platform.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
36036275
Reads aligned (%)
15.7
Duplicates removed (%)
62.2
Number of peaks
5302 (qval < 1E-05)

hg38

Number of total reads
36036275
Reads aligned (%)
16.4
Duplicates removed (%)
60.8
Number of peaks
5292 (qval < 1E-05)

Base call quality data from DBCLS SRA