GSM1577745: ChIPSeq Control STAT5B rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
STAT5B
Cell type
Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
Pre-activated CD8+ T cells
tissue
Peripheral blood
cell type
Pre-activated CD8+ T cells
chip antibody
STAT5B (Invitrogen, 135300, LOT-1477602A)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA-Seq, RNA from 5 donors was pooled, and 1 μg of the pooled RNA was used to synthesize cDNA 220-400 bp fragments were isolated using 2% E-Gel (Invitrogen), ends were repaired, adaptor was added using T4 DNA ligase (New England Biolabs), and amplified for 17 cycles using PE 1.0 and PE 2.0 primers (Illumina) and Phusion High Fidelity PCR Master Mix (New England Biolabs). For ChIP-Seq, Chromatin from 10-20 million cells was sonicated into 250-500 bp fragments, DNA ends were repaired using polynucleotide kinase and Klenow enzyme, and treated with Taq polymerase to generate a protruding 3' “A” nucleotide. DNA fragments of ~250-450 bp were purified from agarose gels and used for cluster generation and sequencing. Antibody against STAT5B was used for immunoprecipitation. PCR products were barcoded (indexed) and sequenced on Illumina HiSeq 2000 platform.