Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Digestive tract

Cell type

GIST48

NA

NA

Sample

source_name

GIST48 Cells

chip antibody

H3K4me1, Abcam, ab8895

tissue

GIST cell line

cell line

GIST48

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

GIST48 cells were crosslinked for 15-minutes in 1% parformaldehyde. Cells were lysed and chromatin sheared using bioruptor. Sheared chromatin was incubated with anti-rabbit IgG dynabeads pre-conjugated with antibody, washed, eluted, reverse cross-linked, and purified. Purified ChIP DNA was blunt-ended, ligated to Solexa adaptors, amplified with 18-cycles of PCR, and sequenced on Solexa Genome Analyzer.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

41405591

Reads aligned (%)

46.4

Duplicates removed (%)

1.9

Number of peaks

16387 (qval < 1E-05)

hg19

Number of total reads

41405591

Reads aligned (%)

46.3

Duplicates removed (%)

2.0

Number of peaks

16408 (qval < 1E-05)