Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
Muscle satellite cells
NA
NA

Attributes by original data submitter

Sample

source_name
Satellite cells
cell type
quiescent satellite cells
strain
C57Bl/6 background
genotype/variation
SIRT1mKO
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIPSeq, lysates were cleared from sonicated nuclei and used for immunoprecipitation. For RNASeq, RNA was extracted according to TRIzol protocol (invitrogen). For ChIPSeq, cells were cross-linked in 1% formaldehyde and lysate to extract nuclei. The chromatin was sonicated to 250 bp fragments. Histone-DNA complexes were isolated with H4K16ac antibody (Millipore, CS204361) or Sirt1 antibody (Upstate, 07-131) Purified DNA was processed according to manufacturer's protocol. For polyA RNA-Seq, Illumina protocol was followed.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
50632838
Reads aligned (%)
96.1
Duplicates removed (%)
10.4
Number of peaks
4750 (qval < 1E-05)

mm9

Number of total reads
50632838
Reads aligned (%)
95.9
Duplicates removed (%)
10.4
Number of peaks
5085 (qval < 1E-05)

Base call quality data from DBCLS SRA