GSM1569873: WT FI Input ChIPSeq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Muscle
Cell type
Muscle satellite cells
NA
NA
Attributes by original data submitter
Sample
source_name
Satellite cells
cell type
quiescent satellite cells
strain
C57Bl/6 background
genotype/variation
WT
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIPSeq, lysates were cleared from sonicated nuclei and used for immunoprecipitation. For RNASeq, RNA was extracted according to TRIzol protocol (invitrogen). For ChIPSeq, cells were cross-linked in 1% formaldehyde and lysate to extract nuclei. The chromatin was sonicated to 250 bp fragments. Histone-DNA complexes were isolated with H4K16ac antibody (Millipore, CS204361) or Sirt1 antibody (Upstate, 07-131) Purified DNA was processed according to manufacturer's protocol. For polyA RNA-Seq, Illumina protocol was followed.