GATA1-deficient cell line with megakaryocyte and erythrocyte differentiation potential
epitope
MigR1-HA-Gata1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Isolated chromatin was sonicated and pre-cleared with isotype-matched mouse non-specific IgG. Chromatin immunprecipitation was carried out with a HA-tag antibody (Santa Crruz Biotechnology, sc-7392). The formaldehyde crosslinks were reversed by heating to 65-degrees overnight. RNA and protein were removed by treatment with RNAse A and Proteinase K, respectively, and the DNA was isolated using the Qiagen PCR Purification Kit. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# IP-102-1001).