Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gata1

Cell type

Cell type Class
Blood
Cell type
G1ME
NA
NA

Attributes by original data submitter

Sample

source_name
G1ME cells
chip antibody
anti-HA
cell line
G1ME
cell type
GATA1-deficient cell line with megakaryocyte and erythrocyte differentiation potential
epitope
MigR1-HA-Gata1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Isolated chromatin was sonicated and pre-cleared with isotype-matched mouse non-specific IgG. Chromatin immunprecipitation was carried out with a HA-tag antibody (Santa Crruz Biotechnology, sc-7392). The formaldehyde crosslinks were reversed by heating to 65-degrees overnight. RNA and protein were removed by treatment with RNAse A and Proteinase K, respectively, and the DNA was isolated using the Qiagen PCR Purification Kit. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# IP-102-1001).

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
54137321
Reads aligned (%)
85.6
Duplicates removed (%)
46.5
Number of peaks
6999 (qval < 1E-05)

mm9

Number of total reads
54137321
Reads aligned (%)
85.3
Duplicates removed (%)
46.5
Number of peaks
7456 (qval < 1E-05)

Base call quality data from DBCLS SRA