Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
RS 411
NA
NA

Attributes by original data submitter

Sample

source_name
B-ALL cell line
tissue
bone marrow

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked in 1% formaldehyde, lysed and then sheared chromatin was immunoprecipitated with antibodies targeting histone marks [H3K36me2 (Abcam, ab9049), H3K27me3 (Abcam, ab6002), H3K27ac (Abcam, ab4729) and H3K9ac (Millipore, 06-942)]. A small aliquot was set aside prior to immunoprecipitation as input DNA. Quality control was performed using an Agilent Technologies 2100 Bioanalyzer prior to library preparation. KAPA HyperPrep Kit was used for library construction

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
21863240
Reads aligned (%)
97.9
Duplicates removed (%)
7.1
Number of peaks
10648 (qval < 1E-05)

hg19

Number of total reads
21863240
Reads aligned (%)
97.3
Duplicates removed (%)
7.8
Number of peaks
10649 (qval < 1E-05)

Base call quality data from DBCLS SRA