Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
reprogramming cells
cell type
TRA1-60+ reprogramming cells
reprogramming factor
OCT6SKM
reprogramming day
2day
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted by reverse crosslinking at 65 °C overnight with proteinase K (20 μg/μl) and RNaseA (20 ug/ul) and purified using phenol/chloroform extraction. Sequencing libraries were prepared using NEBNext Ultra II DNA library prep kit (NEB).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
42854378
Reads aligned (%)
98.1
Duplicates removed (%)
3.1
Number of peaks
1331 (qval < 1E-05)

hg19

Number of total reads
42854378
Reads aligned (%)
97.0
Duplicates removed (%)
3.3
Number of peaks
612 (qval < 1E-05)

Base call quality data from DBCLS SRA