Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
iPSC intermediate cells
cell type
OCT7-transduced iPSC intermediate cells that were cultured with SGC0946
tagged protein
OCT7-FLAG
day of reprogramming
day 8
chip antibody
none (input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Sequencing libraries were prepared using the NEBNext Ultra II DNA library prep kit (NEB).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
36513598
Reads aligned (%)
98.1
Duplicates removed (%)
3.0
Number of peaks
1098 (qval < 1E-05)

hg19

Number of total reads
36513598
Reads aligned (%)
97.0
Duplicates removed (%)
3.1
Number of peaks
543 (qval < 1E-05)

Base call quality data from DBCLS SRA