Antigen

Antigen Class

TFs and others

Antigen

RELA

Cell type

Cell type Class

Adipocyte

Cell type

SGBS

NA

NA

Sample

source_name

Adipocyte

cell type

SGBS adipocyte D10

chip antibody

RELA (C-20, sc-372, Santa Cruz)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments were performed according to standard protocol as described in (Siersbæk et al. 2012, MCB, 32: 3452-3463) Libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014, Methods in Enzymology 2014, Vol. 537, pp. 261-279).

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg19

Number of total reads

22896263

Reads aligned (%)

79.9

Duplicates removed (%)

52.1

Number of peaks

42342 (qval < 1E-05)

hg38

Number of total reads

22896263

Reads aligned (%)

80.3

Duplicates removed (%)

51.6

Number of peaks

42424 (qval < 1E-05)