GSM1566556: ChIP-seq analysis of H3K4me1 in KB cells (H3K4me1 IL1 5Z); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Uterus
Cell type
KB
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
KB cells
treatment_1
10ng/ml IL-1alpha for 1 h
treatment_2
1µM 5Z-7-oxozeaenol for 1 h
antibody
Anti-H3K4me1 (Abcam, catalog# ab8895, Lot GR61280)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP lysis buffer (1% SDS, 10mM EDTA, 50mM Tris pH 8.1, 1mM PMSF, Roche protease inhibitor mix), followed by IP and purification of DNA Sequencing libraries were prepared from 10 ng of immunoprecipitated DNA with the Illumina ChIPSeq DNA Sample Prep Kit according to Illuminas’ instructions